Translocation Assay:Article Title: Amino acids stimulate the endosome-to-Golgi trafficking through Ragulator and small GTPase Arl5
Article Snippet: The following shRNA constructs in pLKO.1 were gifts from D. Sabatini.The following shRNA constructs in pLKO.1 were gifts from D. Sabatini.. Lamtor1 (Addgene: #26631), Lamtor3 (Addgene: #26632), RagA #1 (Addgene: #30319), RagB #1 (Addgene: #26627).. RNAi-resistant Lamtor1 and Arl5b expressing constructs To construct RNAi-resistant Lamtor1 in pLVX-puro vector that can rescue Lamtor1-shRNA mediated knockdown, two PCR amplifications were performed to introduce silent mutations in the shRNA target region, by using Lamtor1 as the template and primer pairs (5’-AGT GAT CTC GAG GCC ACC ATG GGG TGC TGC TAC AG-3’ and 5’-ATT ATG TTG GAC GCC GTC TTG GCA AG G ATG GAA GAG-3’) and (5’-CGG CGT CCA ACA TAA TAG ATG TGT CTG CTG CAG AC-3’ and 5’-AGC GAC GAA TTC TCA TGG GAT CCC AAA CTG TAC-3’).RNAi-resistant Lamtor1 and Arl5b expressing constructs To construct RNAi-resistant Lamtor1 in pLVX-puro vector that can rescue Lamtor1-shRNA mediated knockdown, two PCR amplifications were performed to introduce silent mutations in the shRNA target region, by using Lamtor1 as the template and primer pairs (5’-AGT GAT CTC GAG GCC ACC ATG GGG TGC TGC TAC AG-3’ and 5’-ATT ATG TTG GAC..
Article Title: Amino acids stimulate the endosome-to-Golgi trafficking through Ragulator and small GTPase Arl5
Article Snippet: The following shRNA constructs in pLKO.1 were gifts from D. Sabatini.The following shRNA constructs in pLKO.1 were gifts from D. Sabatini.. Lamtor1 (Addgene: #26631), Lamtor3 (Addgene: #26632), RagA #1 (Addgene: #30319), RagB #1 (Addgene: #26627).
Expressing:Article Title: Amino acids stimulate the endosome-to-Golgi trafficking through Ragulator and small GTPase Arl5
Article Snippet: The following shRNA constructs in pLKO.1 were gifts from D. Sabatini.The following shRNA constructs in pLKO.1 were gifts from D. Sabatini.. Lamtor1 (Addgene: #26631), Lamtor3 (Addgene: #26632), RagA #1 (Addgene: #30319), RagB #1 (Addgene: #26627).. RNAi-resistant Lamtor1 and Arl5b expressing constructs To construct RNAi-resistant Lamtor1 in pLVX-puro vector that can rescue Lamtor1-shRNA mediated knockdown, two PCR amplifications were performed to introduce silent mutations in the shRNA target region, by using Lamtor1 as the template and primer pairs (5’-AGT GAT CTC GAG GCC ACC ATG GGG TGC TGC TAC AG-3’ and 5’-ATT ATG TTG GAC GCC GTC TTG GCA AG G ATG GAA GAG-3’) and (5’-CGG CGT CCA ACA TAA TAG ATG TGT CTG CTG CAG AC-3’ and 5’-AGC GAC GAA TTC TCA TGG GAT CCC AAA CTG TAC-3’).RNAi-resistant Lamtor1 and Arl5b expressing constructs To construct RNAi-resistant Lamtor1 in pLVX-puro vector that can rescue Lamtor1-shRNA mediated knockdown, two PCR amplifications were performed to introduce silent mutations in the shRNA target region, by using Lamtor1 as the template and primer pairs (5’-AGT GAT CTC GAG GCC ACC ATG GGG TGC TGC TAC AG-3’ and 5’-ATT ATG TTG GAC..
Article Title: Amino acids stimulate the endosome-to-Golgi trafficking through Ragulator and small GTPase Arl5
Article Snippet: The following shRNA constructs in pLKO.1 were gifts from D. Sabatini.The following shRNA constructs in pLKO.1 were gifts from D. Sabatini.. Lamtor1 (Addgene: #26631), Lamtor3 (Addgene: #26632), RagA #1 (Addgene: #30319), RagB #1 (Addgene: #26627).
Incubation:Article Title: Amino acids stimulate the endosome-to-Golgi trafficking through Ragulator and small GTPase Arl5
Article Snippet: The following shRNA constructs in pLKO.1 were gifts from D. Sabatini.The following shRNA constructs in pLKO.1 were gifts from D. Sabatini.. Lamtor1 (Addgene: #26631), Lamtor3 (Addgene: #26632), RagA #1 (Addgene: #30319), RagB #1 (Addgene: #26627).. RNAi-resistant Lamtor1 and Arl5b expressing constructs To construct RNAi-resistant Lamtor1 in pLVX-puro vector that can rescue Lamtor1-shRNA mediated knockdown, two PCR amplifications were performed to introduce silent mutations in the shRNA target region, by using Lamtor1 as the template and primer pairs (5’-AGT GAT CTC GAG GCC ACC ATG GGG TGC TGC TAC AG-3’ and 5’-ATT ATG TTG GAC GCC GTC TTG GCA AG G ATG GAA GAG-3’) and (5’-CGG CGT CCA ACA TAA TAG ATG TGT CTG CTG CAG AC-3’ and 5’-AGC GAC GAA TTC TCA TGG GAT CCC AAA CTG TAC-3’).RNAi-resistant Lamtor1 and Arl5b expressing constructs To construct RNAi-resistant Lamtor1 in pLVX-puro vector that can rescue Lamtor1-shRNA mediated knockdown, two PCR amplifications were performed to introduce silent mutations in the shRNA target region, by using Lamtor1 as the template and primer pairs (5’-AGT GAT CTC GAG GCC ACC ATG GGG TGC TGC TAC AG-3’ and 5’-ATT ATG TTG GAC..
Article Title: Amino acids stimulate the endosome-to-Golgi trafficking through Ragulator and small GTPase Arl5
Article Snippet: The following shRNA constructs in pLKO.1 were gifts from D. Sabatini.The following shRNA constructs in pLKO.1 were gifts from D. Sabatini.. Lamtor1 (Addgene: #26631), Lamtor3 (Addgene: #26632), RagA #1 (Addgene: #30319), RagB #1 (Addgene: #26627).
Knockdown:Article Title: Amino acids stimulate the endosome-to-Golgi trafficking through Ragulator and small GTPase Arl5
Article Snippet: The following shRNA constructs in pLKO.1 were gifts from D. Sabatini.The following shRNA constructs in pLKO.1 were gifts from D. Sabatini.. Lamtor1 (Addgene: #26631), Lamtor3 (Addgene: #26632), RagA #1 (Addgene: #30319), RagB #1 (Addgene: #26627).. RNAi-resistant Lamtor1 and Arl5b expressing constructs To construct RNAi-resistant Lamtor1 in pLVX-puro vector that can rescue Lamtor1-shRNA mediated knockdown, two PCR amplifications were performed to introduce silent mutations in the shRNA target region, by using Lamtor1 as the template and primer pairs (5’-AGT GAT CTC GAG GCC ACC ATG GGG TGC TGC TAC AG-3’ and 5’-ATT ATG TTG GAC GCC GTC TTG GCA AG G ATG GAA GAG-3’) and (5’-CGG CGT CCA ACA TAA TAG ATG TGT CTG CTG CAG AC-3’ and 5’-AGC GAC GAA TTC TCA TGG GAT CCC AAA CTG TAC-3’).RNAi-resistant Lamtor1 and Arl5b expressing constructs To construct RNAi-resistant Lamtor1 in pLVX-puro vector that can rescue Lamtor1-shRNA mediated knockdown, two PCR amplifications were performed to introduce silent mutations in the shRNA target region, by using Lamtor1 as the template and primer pairs (5’-AGT GAT CTC GAG GCC ACC ATG GGG TGC TGC TAC AG-3’ and 5’-ATT ATG TTG GAC..
Article Title: Amino acids stimulate the endosome-to-Golgi trafficking through Ragulator and small GTPase Arl5
Article Snippet: The following shRNA constructs in pLKO.1 were gifts from D. Sabatini.The following shRNA constructs in pLKO.1 were gifts from D. Sabatini.. Lamtor1 (Addgene: #26631), Lamtor3 (Addgene: #26632), RagA #1 (Addgene: #30319), RagB #1 (Addgene: #26627).
Control:Article Title: Amino acids stimulate the endosome-to-Golgi trafficking through Ragulator and small GTPase Arl5
Article Snippet: The following shRNA constructs in pLKO.1 were gifts from D. Sabatini.The following shRNA constructs in pLKO.1 were gifts from D. Sabatini.. Lamtor1 (Addgene: #26631), Lamtor3 (Addgene: #26632), RagA #1 (Addgene: #30319), RagB #1 (Addgene: #26627).. RNAi-resistant Lamtor1 and Arl5b expressing constructs To construct RNAi-resistant Lamtor1 in pLVX-puro vector that can rescue Lamtor1-shRNA mediated knockdown, two PCR amplifications were performed to introduce silent mutations in the shRNA target region, by using Lamtor1 as the template and primer pairs (5’-AGT GAT CTC GAG GCC ACC ATG GGG TGC TGC TAC AG-3’ and 5’-ATT ATG TTG GAC GCC GTC TTG GCA AG G ATG GAA GAG-3’) and (5’-CGG CGT CCA ACA TAA TAG ATG TGT CTG CTG CAG AC-3’ and 5’-AGC GAC GAA TTC TCA TGG GAT CCC AAA CTG TAC-3’).RNAi-resistant Lamtor1 and Arl5b expressing constructs To construct RNAi-resistant Lamtor1 in pLVX-puro vector that can rescue Lamtor1-shRNA mediated knockdown, two PCR amplifications were performed to introduce silent mutations in the shRNA target region, by using Lamtor1 as the template and primer pairs (5’-AGT GAT CTC GAG GCC ACC ATG GGG TGC TGC TAC AG-3’ and 5’-ATT ATG TTG GAC..
Article Title: Amino acids stimulate the endosome-to-Golgi trafficking through Ragulator and small GTPase Arl5
Article Snippet: The following shRNA constructs in pLKO.1 were gifts from D. Sabatini.The following shRNA constructs in pLKO.1 were gifts from D. Sabatini.. Lamtor1 (Addgene: #26631), Lamtor3 (Addgene: #26632), RagA #1 (Addgene: #30319), RagB #1 (Addgene: #26627).
shRNA:Article Title: Amino acids stimulate the endosome-to-Golgi trafficking through Ragulator and small GTPase Arl5
Article Snippet: The following shRNA constructs in pLKO.1 were gifts from D. Sabatini.The following shRNA constructs in pLKO.1 were gifts from D. Sabatini.. Lamtor1 (Addgene: #26631), Lamtor3 (Addgene: #26632), RagA #1 (Addgene: #30319), RagB #1 (Addgene: #26627).. RNAi-resistant Lamtor1 and Arl5b expressing constructs To construct RNAi-resistant Lamtor1 in pLVX-puro vector that can rescue Lamtor1-shRNA mediated knockdown, two PCR amplifications were performed to introduce silent mutations in the shRNA target region, by using Lamtor1 as the template and primer pairs (5’-AGT GAT CTC GAG GCC ACC ATG GGG TGC TGC TAC AG-3’ and 5’-ATT ATG TTG GAC GCC GTC TTG GCA AG G ATG GAA GAG-3’) and (5’-CGG CGT CCA ACA TAA TAG ATG TGT CTG CTG CAG AC-3’ and 5’-AGC GAC GAA TTC TCA TGG GAT CCC AAA CTG TAC-3’).RNAi-resistant Lamtor1 and Arl5b expressing constructs To construct RNAi-resistant Lamtor1 in pLVX-puro vector that can rescue Lamtor1-shRNA mediated knockdown, two PCR amplifications were performed to introduce silent mutations in the shRNA target region, by using Lamtor1 as the template and primer pairs (5’-AGT GAT CTC GAG GCC ACC ATG GGG TGC TGC TAC AG-3’ and 5’-ATT ATG TTG GAC..
Article Title: Amino acids stimulate the endosome-to-Golgi trafficking through Ragulator and small GTPase Arl5
Article Snippet: The following shRNA constructs in pLKO.1 were gifts from D. Sabatini.The following shRNA constructs in pLKO.1 were gifts from D. Sabatini.. Lamtor1 (Addgene: #26631), Lamtor3 (Addgene: #26632), RagA #1 (Addgene: #30319), RagB #1 (Addgene: #26627).
Quantitative RT-PCR:Article Title: Amino acids stimulate the endosome-to-Golgi trafficking through Ragulator and small GTPase Arl5
Article Snippet: The following shRNA constructs in pLKO.1 were gifts from D. Sabatini.The following shRNA constructs in pLKO.1 were gifts from D. Sabatini.. Lamtor1 (Addgene: #26631), Lamtor3 (Addgene: #26632), RagA #1 (Addgene: #30319), RagB #1 (Addgene: #26627).. RNAi-resistant Lamtor1 and Arl5b expressing constructs To construct RNAi-resistant Lamtor1 in pLVX-puro vector that can rescue Lamtor1-shRNA mediated knockdown, two PCR amplifications were performed to introduce silent mutations in the shRNA target region, by using Lamtor1 as the template and primer pairs (5’-AGT GAT CTC GAG GCC ACC ATG GGG TGC TGC TAC AG-3’ and 5’-ATT ATG TTG GAC GCC GTC TTG GCA AG G ATG GAA GAG-3’) and (5’-CGG CGT CCA ACA TAA TAG ATG TGT CTG CTG CAG AC-3’ and 5’-AGC GAC GAA TTC TCA TGG GAT CCC AAA CTG TAC-3’).RNAi-resistant Lamtor1 and Arl5b expressing constructs To construct RNAi-resistant Lamtor1 in pLVX-puro vector that can rescue Lamtor1-shRNA mediated knockdown, two PCR amplifications were performed to introduce silent mutations in the shRNA target region, by using Lamtor1 as the template and primer pairs (5’-AGT GAT CTC GAG GCC ACC ATG GGG TGC TGC TAC AG-3’ and 5’-ATT ATG TTG GAC..
Article Title: Amino acids stimulate the endosome-to-Golgi trafficking through Ragulator and small GTPase Arl5
Article Snippet: The following shRNA constructs in pLKO.1 were gifts from D. Sabatini.The following shRNA constructs in pLKO.1 were gifts from D. Sabatini.. Lamtor1 (Addgene: #26631), Lamtor3 (Addgene: #26632), RagA #1 (Addgene: #30319), RagB #1 (Addgene: #26627).
Activity Assay:Article Title: Amino acids stimulate the endosome-to-Golgi trafficking through Ragulator and small GTPase Arl5
Article Snippet: The following shRNA constructs in pLKO.1 were gifts from D. Sabatini.The following shRNA constructs in pLKO.1 were gifts from D. Sabatini.. Lamtor1 (Addgene: #26631), Lamtor3 (Addgene: #26632), RagA #1 (Addgene: #30319), RagB #1 (Addgene: #26627).. RNAi-resistant Lamtor1 and Arl5b expressing constructs To construct RNAi-resistant Lamtor1 in pLVX-puro vector that can rescue Lamtor1-shRNA mediated knockdown, two PCR amplifications were performed to introduce silent mutations in the shRNA target region, by using Lamtor1 as the template and primer pairs (5’-AGT GAT CTC GAG GCC ACC ATG GGG TGC TGC TAC AG-3’ and 5’-ATT ATG TTG GAC GCC GTC TTG GCA AG G ATG GAA GAG-3’) and (5’-CGG CGT CCA ACA TAA TAG ATG TGT CTG CTG CAG AC-3’ and 5’-AGC GAC GAA TTC TCA TGG GAT CCC AAA CTG TAC-3’).RNAi-resistant Lamtor1 and Arl5b expressing constructs To construct RNAi-resistant Lamtor1 in pLVX-puro vector that can rescue Lamtor1-shRNA mediated knockdown, two PCR amplifications were performed to introduce silent mutations in the shRNA target region, by using Lamtor1 as the template and primer pairs (5’-AGT GAT CTC GAG GCC ACC ATG GGG TGC TGC TAC AG-3’ and 5’-ATT ATG TTG GAC..
Article Title: Amino acids stimulate the endosome-to-Golgi trafficking through Ragulator and small GTPase Arl5
Article Snippet: The following shRNA constructs in pLKO.1 were gifts from D. Sabatini.The following shRNA constructs in pLKO.1 were gifts from D. Sabatini.. Lamtor1 (Addgene: #26631), Lamtor3 (Addgene: #26632), RagA #1 (Addgene: #30319), RagB #1 (Addgene: #26627).
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